ADAM9 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6819
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6819-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ADAM9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat lung, 2) rat liver, 3) rat heart, 4) rat C6, 5) mouse lung, 6) mouse liver, 7) mouse heart and 8) mouse C2C12 cell lysate with ADAM9 antibody. Expected molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
2 / 3
Western blot testing of human 1) U-87 MG, 2) A431, 3) A549, 4) U-251, 5) U-2 OS, 6) HeLa, 7) HaCat and 8) SiHa cell lysate with ADAM9 antibody. Expected molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
3 / 3
Flow cytometry testing of human U-251 cells with ADAM9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAM9 antibody.
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Western blot testing of 1) rat lung, 2) rat liver, 3) rat heart, 4) rat C6, 5) mouse lung, 6) mouse liver, 7) mouse heart and 8) mouse C2C12 cell lysate with ADAM9 antibody. Expected molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
Western blot testing of human 1) U-87 MG, 2) A431, 3) A549, 4) U-251, 5) U-2 OS, 6) HeLa, 7) HaCat and 8) SiHa cell lysate with ADAM9 antibody. Expected molecular weight: 90-100 kDa (pro-ADAM9-L form) and 70-80 kDa (ADMA9-L form).
Flow cytometry testing of human U-251 cells with ADAM9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ADAM9 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ADAM9 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Disintegrin and metalloproteinase domain-containing protein 9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A29-K307) was used as the immunogen for the ADAM9 antibody.
Limitation:
This ADAM9 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q13443