Azurocidin Antibody / AZU1 / CAP37 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6825
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6825-100ug100ug£535.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Azurocidin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human appendicitis tissue with Azurocidin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen, 4) mouse thymus and 5) mouse RAW264.7 cell lysate with Azurocidin antibody. Expected molecular weight: 27-37 kDa.
3 / 3
Flow cytometry testing of human ThP-1 cells with Azurocidin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Azurocidin antibody.
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IHC staining of FFPE human appendicitis tissue with Azurocidin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen, 4) mouse thymus and 5) mouse RAW264.7 cell lysate with Azurocidin antibody. Expected molecular weight: 27-37 kDa.
Flow cytometry testing of human ThP-1 cells with Azurocidin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Azurocidin antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Azurocidin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Azurocidin also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP) is a protein that in humans is encoded by the AZU1 gene. Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. This gene encodes a preproprotein that is proteolytically processed to generate a mature azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids I27-N245) was used as the immunogen for the Azurocidin antibody.
Limitation:
This Azurocidin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P20160