BRD4 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6828
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6828-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Bromodomain-containing protein 4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human PC-3 cells with Bromodomain-containing protein 4 antibody.
2 / 4
Western blot testing of human 1) 293T, 2) Jurkat, 3) K562, 4) MCF7, 5) PC-3 and 6) HL60 cell lysate with Bromodomain-containing protein 4 antibody. Predicted molecular weight ~156 kDa (long form, can be observed at 200+ kDa) and ~81 kDa (short form).
3 / 4
Flow cytometry testing of human U-2 OS cells with Bromodomain-containing protein 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bromodomain-containing protein 4 antibody.
4 / 4
Flow cytometry testing of rat C6 cells with Bromodomain-containing protein 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bromodomain-containing protein 4 antibody.
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Immunofluorescent staining of FFPE human PC-3 cells with Bromodomain-containing protein 4 antibody.
Western blot testing of human 1) 293T, 2) Jurkat, 3) K562, 4) MCF7, 5) PC-3 and 6) HL60 cell lysate with Bromodomain-containing protein 4 antibody. Predicted molecular weight ~156 kDa (long form, can be observed at 200+ kDa) and ~81 kDa (short form).
Flow cytometry testing of human U-2 OS cells with Bromodomain-containing protein 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bromodomain-containing protein 4 antibody.
Flow cytometry testing of rat C6 cells with Bromodomain-containing protein 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bromodomain-containing protein 4 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Bromodomain-containing protein 4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Bromodomain-containing protein 4 is a protein that in humans is encoded by the BRD4 gene. The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QRPEMKPVDVGRPVIRPPEQ from the human protein were used as the immunogen for the Bromodomain-containing protein 4 antibody.
Limitation:
This Bromodomain-containing protein 4 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
O60885