Caspase-8 Antibody (large subunit) (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6832
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6832-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Caspase-8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-251 cells with Caspase-8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase-8 antibody.~
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Western blot testing of human 1) HepG2, 2) Caco-2, 3) PANC-1, 4)
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Flow cytometry testing of human U-251 cells with Caspase-8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase-8 antibody.~
Western blot testing of human 1) HepG2, 2) Caco-2, 3) PANC-1, 4)

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Caspase-8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids HFEIKPHDDCTVEQIYE from the human protein were used as the immunogen for the Caspase-8 antibody.
Limitation:
This Caspase-8 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q14790