p19-INK4d Antibody / CDKN2D (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6844
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6844-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CDKN2D antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-87 MG cells with CDKN2D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDKN2D antibody.~
2 / 2
Western blot testing of human 1) MOLT4 and 2) HEL cell lysate with CD
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Flow cytometry testing of human U-87 MG cells with CDKN2D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDKN2D antibody.~
Western blot testing of human 1) MOLT4 and 2) HEL cell lysate with CD

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDKN2D antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cyclin-dependent kinase 4 inhibitor D is an enzyme that in humans is encoded by the CDKN2D gene. The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E31-H132) was used as the immunogen for the CDKN2D antibody.
Limitation:
This CDKN2D antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P55273