Calmegin Antibody / CLGN (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6849
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6849-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Calmegin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human ovarian cancer tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human breast cancer tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse testis tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat testis tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human Jurkat, 2) human HepG2, 3) human PC-2, 4) human Raji, 5) rat testis and 6) mouse testis tissue lysate with Calmegin antibody. Predicted molecular weight ~70 kDa but can be observed at ~93 kDa with an ~101 kDa phosphorylated form.
6 / 6
Flow cytometry testing of human Jurkat cells with Calmegin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calmegin antibody.
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IHC staining of FFPE human ovarian cancer tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with Calmegin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human HepG2, 3) human PC-2, 4) human Raji, 5) rat testis and 6) mouse testis tissue lysate with Calmegin antibody. Predicted molecular weight ~70 kDa but can be observed at ~93 kDa with an ~101 kDa phosphorylated form.
Flow cytometry testing of human Jurkat cells with Calmegin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calmegin antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Calmegin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Calmegin, also known as CLGN, is a protein which in humans is encoded by the CLGN gene. Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E36-D610) was used as the immunogen for the Calmegin antibody.
Limitation:
This Calmegin antibody is available for research use only.
Localization:
Cytoplasmic (ER)
Purity:
Antigen affinity purified
Uniprot #:
O14967