Cathepsin L Antibody / Ctsl (Antigen affinity purified)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6858
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6858-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cathepsin L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE rat kidney tissue with Cathepsin L antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat liver, 2) rat kidney and 3) mouse liver tissue lysate with Cathepsin L antibody. Expected molecular weight: 38-41 kDa with multiple smaller processed/active forms.
3 / 3
Flow cytometry testing of mouse HEPA1-6 cells with Cathepsin L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cathepsin L antibody.
❮❯

IHC staining of FFPE rat kidney tissue with Cathepsin L antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat liver, 2) rat kidney and 3) mouse liver tissue lysate with Cathepsin L antibody. Expected molecular weight: 38-41 kDa with multiple smaller processed/active forms.
Flow cytometry testing of mouse HEPA1-6 cells with Cathepsin L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cathepsin L antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Cathepsin L antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KYRAEFAVANDTGFVDIPQQ from the mouse protein were used as the immunogen for the Cathepsin L antibody.
Limitation:
This Cathepsin L antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P06797