Gastrin Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6873
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6873-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the GAST antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human Caco-2 cells with GAST antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GAST antibody.
2 / 3
Flow cytometry testing of human U-87 MG cells with GAST antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GAST antibody.
3 / 3
Western blot testing of 1) human Caco-2 and 2) rat stomach tissue lysate with GAST antibody. Predicted molecular weight ~11 kDa.
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Flow cytometry testing of human Caco-2 cells with GAST antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GAST antibody.
Flow cytometry testing of human U-87 MG cells with GAST antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GAST antibody.
Western blot testing of 1) human Caco-2 and 2) rat stomach tissue lysate with GAST antibody. Predicted molecular weight ~11 kDa.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the GAST antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RDLELPWLEQQGPASHHRRQL from the human protein were used as the immunogen for the GAST antibody.
Limitation:
This GAST antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P01350