hHR23A Antibody / RAD23A (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6895
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6895-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the hHR23A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HeLa cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
2 / 4
Flow cytometry testing of mouse ANA-1 cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
3 / 4
Flow cytometry testing of rat C6 cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
4 / 4
Western blot testing of 1) human K562, 2) human HeLa, 3) human MCF7, 4) human Jurkat, 5) rat testis, 6) mouse testis and 7) mouse lung tissue lysate with hHR23A antibody. Predicted molecular weight ~40 kDa but commonly observed at 40-55 kDa and possibly larger due to ubiquitination.
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Flow cytometry testing of human HeLa cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
Flow cytometry testing of mouse ANA-1 cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
Flow cytometry testing of rat C6 cells with hHR23A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= hHR23A antibody.
Western blot testing of 1) human K562, 2) human HeLa, 3) human MCF7, 4) human Jurkat, 5) rat testis, 6) mouse testis and 7) mouse lung tissue lysate with hHR23A antibody. Predicted molecular weight ~40 kDa but commonly observed at 40-55 kDa and possibly larger due to ubiquitination.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the hHR23A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
UV excision repair protein RAD23 homolog A is a protein that in humans is encoded by the RAD23A gene. The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KIRMEPDETVKVLKEKIEAEK from the human protein were used as the immunogen for the hHR23A antibody.
Limitation:
This hHR23A antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P54725