SLC25A51/52 Antibody / MCART1/2 (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6906
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6906-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MCART1/2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MCART1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human serous adenocarcinoma of ovary tissue with MCART1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HeLa, 2) monkey COS-7 and 3) mouse NIH 3T3 cell lysate with MCART1/2 antibody. Predicted molecular weight ~34 kDa.
4 / 4
Flow cytometry testing of human U-251 cells with MCART1/2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCART1/2 antibody.

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MCART1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human serous adenocarcinoma of ovary tissue with MCART1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) monkey COS-7 and 3) mouse NIH 3T3 cell lysate with MCART1/2 antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human U-251 cells with MCART1/2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCART1/2 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MCART1/2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SLC25A51 gene ontology annotations related to this gene include transmembrane transporter activity.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-E291) was used as the immunogen for the MCART1/2 antibody.
Limitation:
This MCART1/2 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
Q9H1U9, Q3SY17