SP6 Antibody / Transcription factor Sp6 / KLF14 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6907
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6907-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the KLF14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human Caco-2 cells with KLF14 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human placenta, 2) human Caco-2, 3) human HeLa, 4) rat brain, 5) rat C6, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with KLF14 antibody. Predicted molecular weight ~40 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with KLF14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLF14 antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with KLF14 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human placenta, 2) human Caco-2, 3) human HeLa, 4) rat brain, 5) rat C6, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with KLF14 antibody. Predicted molecular weight ~40 kDa.
Flow cytometry testing of human Caco-2 cells with KLF14 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KLF14 antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KLF14 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S11-S218) was used as the immunogen for the KLF14 antibody.
Limitation:
This KLF14 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q3SY56