GDP-L-fucose synthase Antibody / GFUS / TSTA3 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6910
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6910-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the GDP-L-fucose synthase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with GDP-L-fucose synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human MCF7, 2) human HeLa, 3) human HepG2, 4) human RT-4, 5) human PC-3, 6) rat testis, 7) rat kidney, 8) rat brain, 9) mouse testis and 10) mouse kidney tissue lysate with GDP-L-fucose synthase antibody. Predicted molecular weight ~36 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with GDP-L-fucose synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GDP-L-fucose synthase antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with GDP-L-fucose synthase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human MCF7, 2) human HeLa, 3) human HepG2, 4) human RT-4, 5) human PC-3, 6) rat testis, 7) rat kidney, 8) rat brain, 9) mouse testis and 10) mouse kidney tissue lysate with GDP-L-fucose synthase antibody. Predicted molecular weight ~36 kDa.
Flow cytometry testing of human U-87 MG cells with GDP-L-fucose synthase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GDP-L-fucose synthase antibody.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GDP-L-fucose synthase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K44-K321) was used as the immunogen for the GDP-L-fucose synthase antibody.
Limitation:
This GDP-L-fucose synthase antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q13630