Egf Receptor / Egfr Antibody (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6913
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6913-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Egf receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE mouse liver with Egf receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat liver with Egf receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) rat liver, 2) mouse liver, 3) mouse spleen and 4) mouse lung tissue lysate with Egf receptor antibody. Expected molecular weight: 134-180 kDa depending on glycosylation level.

IHC staining of FFPE mouse liver with Egf receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with Egf receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat liver, 2) mouse liver, 3) mouse spleen and 4) mouse lung tissue lysate with Egf receptor antibody. Expected molecular weight: 134-180 kDa depending on glycosylation level.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml
Application Note:
Optimal dilution of the Egf receptor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. It is mapped to 11 A2; 11 9.41 cM. The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids L25-P596) was used as the immunogen for the Egf receptor antibody.
Limitation:
This Egf receptor antibody is available for research use only.
Purity:
Affinity purified
Uniprot #:
Q01279