P Glycoprotein Antibody / MDR1 / ABCB1 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6915
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6915-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the P Glycoprotein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human Caco-2 cells with P Glycoprotein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= P Glycoprotein antibody.
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Western blot testing of human 1) DLD1 and 2) HUH-7 cell lysate with P Glycoprotein antibody. Expected molecular weight: 141-180 kDa depending on glycosylation level.
3 / 4
IHC staining of FFPE human thyroid cancer tissue with P Glycoprotein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE human liver cancer tissue with P Glycoprotein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human Caco-2 cells with P Glycoprotein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= P Glycoprotein antibody.
Western blot testing of human 1) DLD1 and 2) HUH-7 cell lysate with P Glycoprotein antibody. Expected molecular weight: 141-180 kDa depending on glycosylation level.
IHC staining of FFPE human thyroid cancer tissue with P Glycoprotein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with P Glycoprotein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the P Glycoprotein antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood�brain barrier.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human P Glycoprotein/ABCB1 protein (amino acids M1-E690) was used as the immunogen for the P Glycoprotein antibody.
Limitation:
This P Glycoprotein antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P08183