RORB Antibody / ROR beta (Antigen affinity purified)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6921
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6921-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ROR beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of mouse Neuro-2a cells with ROR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROR beta antibody.
2 / 3
Flow cytometry testing of rat RH35 cells with ROR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROR beta antibody.
3 / 3
Western blot testing of 1) human HepG2, 2) mouse brain and 3) rat brain tissue lysate with ROR beta antibody. Predicted molecular weight ~53 kDa.
❮❯

Flow cytometry testing of mouse Neuro-2a cells with ROR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROR beta antibody.
Flow cytometry testing of rat RH35 cells with ROR beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ROR beta antibody.
Western blot testing of 1) human HepG2, 2) mouse brain and 3) rat brain tissue lysate with ROR beta antibody. Predicted molecular weight ~53 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ROR beta antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RAR-related orphan receptor beta (ROR-beta), also known as NR1F2 (nuclear receptor subfamily 1, group F, member 2) is a nuclear receptor that in humans is encoded by the RORB gene. The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It is a DNA-binding protein that can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, and to help regulate the expression of some genes involved in circadian rhythm.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human ROR beta/RORB protein (amino acids M1-K470) was used as the immunogen for the ROR beta antibody.
Limitation:
This ROR beta antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q92753