SERPINB5 Antibody / MASPIN [Clone 7G4E1] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6922
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6922-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 7G4E1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MASPIN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human tonsil tissue with MASPIN antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MASPIN antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) HeLa, 2) HaCaT and 3) Caco-2 cell lysate with MASPIN antibody. Predicted molecular weight: 42~45 kDa.
4 / 4
Flow cytometry testing of human SiHa cells with MASPIN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASPIN antibody.
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IHC staining of FFPE human tonsil tissue with MASPIN antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MASPIN antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HaCaT and 3) Caco-2 cell lysate with MASPIN antibody. Predicted molecular weight: 42~45 kDa.
Flow cytometry testing of human SiHa cells with MASPIN antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASPIN antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MASPIN antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human MASPIN protein (amino acids M1-A350) was used as the immunogen for the MASPIN antibody.
Limitation:
This MASPIN antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P36952