HRS Antibody / HGS / HGF-regulated tyrosine kinase substrate [Clone 4B7E2] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6925
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6925-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 4B7E2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HGF-regulated tyrosine kinase substrate antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-87 MG cells with HGF-regulated tyrosine kinase substrate antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 10
IHC staining of FFPE human endometrial cancer tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human ovarian cancer tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human bladder epithelial carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human colorectal adenocarcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE mouse kidney tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
Western blot testing of 1) human HeLa, 2) human 293T, 3) human K562, 4) human SK-O-V3, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with HGF-regulated tyrosine kinase substrate antibody. Predicted molecular weight ~86 kDa, commonly observed at 110-115 kDa.
10 / 10
Flow cytometry testing of human Caco-2 cells with HGF-regulated tyrosine kinase substrate antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGF-regulated tyrosine kinase substrate antibody.
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Immunofluorescent staining of FFPE human U-87 MG cells with HGF-regulated tyrosine kinase substrate antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human endometrial cancer tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder epithelial carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human K562, 4) human SK-O-V3, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with HGF-regulated tyrosine kinase substrate antibody. Predicted molecular weight ~86 kDa, commonly observed at 110-115 kDa.
Flow cytometry testing of human Caco-2 cells with HGF-regulated tyrosine kinase substrate antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGF-regulated tyrosine kinase substrate antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the HGF-regulated tyrosine kinase substrate antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Hepatocyte growth factor-regulated tyrosine kinase substrate is an enzyme that in humans is encoded by the HGS gene. It is mapped to 17q25.3. The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human HGS protein (amino acids R3-D777) was used as the immunogen for the HGF-regulated tyrosine kinase substrate antibody.
Limitation:
This HGF-regulated tyrosine kinase substrate antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O14964