14-3-3 epsilon Antibody / YWHAE [Clone 3G11G2] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6927
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6927-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3G11G2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the YWHAE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 12
IHC staining of FFPE human bladder epithelial carcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE human tonsil tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE human liver cancer tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human endometrial cancer tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human placental tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human colorectal adenocarcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE rat brain tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
Immunofluorescent staining of FFPE human Caco-2 cells with YWHAE antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 12
Western blot testing of 1) human HeLa, 2) human SH-SY5Y, 3) human SW620, 4) human Raji, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with YWHAE antibody. Predicted molecular weight ~28 kDa.
11 / 12
Flow cytometry testing of mouse ANA-1 cells with YWHAE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YWHAE antibody.
12 / 12
Flow cytometry testing of rat NRK cells with YWHAE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YWHAE antibody.
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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder epithelial carcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial cancer tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with YWHAE antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human SH-SY5Y, 3) human SW620, 4) human Raji, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with YWHAE antibody. Predicted molecular weight ~28 kDa.
Flow cytometry testing of mouse ANA-1 cells with YWHAE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YWHAE antibody.
Flow cytometry testing of rat NRK cells with YWHAE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YWHAE antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the YWHAE antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human YWHAE protein (amino acids M1-Q255) was used as the immunogen for the YWHAE antibody.
Limitation:
This YWHAE antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P62258