Placental Alkaline Phosphatase Antibody / ALPP [Clone 6C7G3] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6933
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6933-100ug100 ug£535.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6C7G3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Placental Alkaline Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human placental tissue with Placental Alkaline Phosphatase antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
Immunofluorescent staining of FFPE human HeLa cells with Placental Alkaline Phosphatase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
IHC staining of FFPE human placental tissue with Placental Alkaline Phosphatase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) placenta, 2) HepG2, 3) A431, 4) HeLa and 5) SW620 cell lysate with Placental Alkaline Phosphatase antibody. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.
5 / 5
Flow cytometry testing of human SiHa cells with Placental Alkaline Phosphatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Placental Alkaline Phosphatase antibody.
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Immunofluorescent staining of FFPE human placental tissue with Placental Alkaline Phosphatase antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with Placental Alkaline Phosphatase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with Placental Alkaline Phosphatase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) HepG2, 3) A431, 4) HeLa and 5) SW620 cell lysate with Placental Alkaline Phosphatase antibody. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.
Flow cytometry testing of human SiHa cells with Placental Alkaline Phosphatase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Placental Alkaline Phosphatase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Placental Alkaline Phosphatase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
C-terminal amino acids KARDRKAYTVLLYGNGPGYVLKDGARPDVTE were used as the immunogen for the Placental Alkaline Phosphatase antibody.
Limitation:
This Placental Alkaline Phosphatase antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Antigen affinity purified
Uniprot #:
P05187