ABP1 Antibody / Amiloride binding protein 1 / AOC1 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6936
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6936-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Amiloride binding protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human T-47D cells with Amiloride binding protein 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human colorectal adenocarcinoma tissue with Amiloride binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human 293T, 2) human HK-2 and 3) monkey COS-7 cell lysate with Amiloride binding protein 1 antibody. Predicted molecular weight ~85 kDa.
4 / 4
Flow cytometry testing of human ThP-1 cells with Amiloride binding protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Amiloride binding protein 1 antibody.
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Immunofluorescent staining of FFPE human T-47D cells with Amiloride binding protein 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Amiloride binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293T, 2) human HK-2 and 3) monkey COS-7 cell lysate with Amiloride binding protein 1 antibody. Predicted molecular weight ~85 kDa.
Flow cytometry testing of human ThP-1 cells with Amiloride binding protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Amiloride binding protein 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Amiloride binding protein 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S140-R180) was used as the immunogen for the Amiloride binding protein 1 antibody.
Limitation:
This Amiloride binding protein 1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Uniprot #:
P19801