LARG Antibody / ARHGEF12 (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ6941
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6941-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the LARG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 2
Western blot testing of 1) human HEL, 2) human K562 and 3) rat brain lysate with LARG antibody. Predicted molecular weight ~173 kDa but commonly observed at up to 220 kDa, possibly due to phosphorylation.~
2 / 2
Flow cytometry testing of human HL60 cells

Western blot testing of 1) human HEL, 2) human K562 and 3) rat brain lysate with LARG antibody. Predicted molecular weight ~173 kDa but commonly observed at up to 220 kDa, possibly due to phosphorylation.~
Flow cytometry testing of human HL60 cells

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LARG antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids T4-S254) was used as the immunogen for the LARG antibody.
Limitation:
This LARG antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9NZN5