CD147 / Emmprin / Basigin Antibody [Clone 6H2B2] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6951
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6951-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6H2B2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Basigin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human esophageal squamous carcinoma tissue with Basigin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 8
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human liver cancer tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human colorectal adenocarcinoma tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human ovarian cancer tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
IHC staining of FFPE mouse kidney tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 8
Western blot testing of human 1) HeLa, 2) Jurkat and 3) HepG2 cell lysate with Basigin antibody. Expected molecular weight: 27-66 kDa depending on level of glycosylation.
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Immunofluorescent staining of FFPE human esophageal squamous carcinoma tissue with Basigin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with Basigin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Jurkat and 3) HepG2 cell lysate with Basigin antibody. Expected molecular weight: 27-66 kDa depending on level of glycosylation.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the Basigin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Emmprin, extracellular matrix metalloproteinase inducer, also known as Basigin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the BSG gene. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. BSG has been shown to be an essential receptor on red blood cells for the malaria parasite. It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily, it plays fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development. BSG is thought also to play a role in intercellular recognition. It also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. BSG is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E138-A323) was used as the immunogen for the Basigin antibody.
Limitation:
This Basigin antibody is available for research use only.
Localization:
Cell surface
Purity:
Antigen affinity purified
Uniprot #:
P35613