CBL Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6954
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6954-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CBL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of human U-87 MG cells with CBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBL antibody.
2 / 3
Western blot testing of 1) human HL60, 2) human K562, 3) human A549, 4) human Raji, 5) human CCRF-CEM, 6) human Daudi, 7) human MCF7, 8) human HeLa, 9) rat testis and 10) mouse ANA-1 cell lysate with CBL antibody. Expected molecular weight: 100-120 kDa.
3 / 3
IHC staining of FFPE human colorectal cancer tissue with CBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human U-87 MG cells with CBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBL antibody.
Western blot testing of 1) human HL60, 2) human K562, 3) human A549, 4) human Raji, 5) human CCRF-CEM, 6) human Daudi, 7) human MCF7, 8) human HeLa, 9) rat testis and 10) mouse ANA-1 cell lysate with CBL antibody. Expected molecular weight: 100-120 kDa.
IHC staining of FFPE human colorectal cancer tissue with CBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CBL antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CBL (Cbl proto-oncogene) is also known as C-CBL, RNF55, CBL2 and E3 ubiquitin-protein ligase CBL. CBL is mapped to chromosome 11q23.3-qter by molecular characterization of the breakpoints in 2 somatic cell hybrids. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes(E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M470-E766) was used as the immunogen for the CBL antibody.
Limitation:
This CBL antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P22681