Sorbitol Dehydrogenase Antibody [Clone 12B10G2] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6958
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6958-100ug100 ug£535.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 12B10G2
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Sorbitol Dehydrogenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human liver cancer tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human SM fatty carcinoma of the left kidney tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human renal clear cell carcinoma tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat kidney tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human HeLa, 2) human HepG2, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with Sorbitol Dehydrogenase antibody. Expected molecular weight: 38-42 kDa.
6 / 6
Flow cytometry testing of human U-2 OS cells with Sorbitol Dehydrogenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sorbitol Dehydrogenase antibody.
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IHC staining of FFPE human liver cancer tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human SM fatty carcinoma of the left kidney tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal clear cell carcinoma tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with Sorbitol Dehydrogenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with Sorbitol Dehydrogenase antibody. Expected molecular weight: 38-42 kDa.
Flow cytometry testing of human U-2 OS cells with Sorbitol Dehydrogenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Sorbitol Dehydrogenase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Sorbitol Dehydrogenase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Sorbitol dehydrogenase is an enzyme that in humans is encoded by the SORD gene. Sorbitol dehydrogenase (SORD) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human Sorbitol Dehydrogenase/SORD protein (amino acids N8-P357) was used as the immunogen for the Sorbitol Dehydrogenase antibody.
Limitation:
This Sorbitol Dehydrogenase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q00796