PPAR gamma Antibody / PPARG (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6963
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6963-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PPARG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of human A431 cells with PPARG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPARG antibody.
2 / 3
Western blot testing of human 1) placenta, 2) MCF7, 3) SK-O-V3 and 4) MDA-MB-453 cell lysate with PPARG antibody. Predicted molecular weight: 54-57 kDa, observed here at ~67 kDa.
3 / 3
Immunofluorescent staining of FFPE human A431 cells with PPARG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human A431 cells with PPARG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPARG antibody.
Western blot testing of human 1) placenta, 2) MCF7, 3) SK-O-V3 and 4) MDA-MB-453 cell lysate with PPARG antibody. Predicted molecular weight: 54-57 kDa, observed here at ~67 kDa.
Immunofluorescent staining of FFPE human A431 cells with PPARG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PPARG antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Peroxisome proliferator- activated receptor gamma (PPAR-gamma or PPARG), also known as the glitazone reverse insulin resistance receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3) is a type II nuclear receptor (protein regulating genes) that in humans is encoded by the PPARG gene. This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H58-K329) was used as the immunogen for the PPARG antibody.
Limitation:
This PPARG antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P37231