Cd70 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6964
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6964-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cd70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of mouse 1) spleen, 2) thymus, 3) lung, 4) RAW264.7, 5) Ana-1 and 6) SP2/0 cell lysate with Cd70 antibody. Predicted molecular weight ~21 kDa but may be observed at up to ~80 kDa due to glycosylation.
2 / 4
Western blot testing of 1) rat spleen, 2) rat thymus, 3) rat lung, 4) rat L6, 5) rat PC-12 and 6) mouse B16 cell lysate with Cd70 antibody. Predicted molecular weight ~21 kDa but may be observed at up to ~80 kDa due to glycosylation.
3 / 4
IHC staining of FFPE mouse spleen tissue with Cd70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE mouse lymph node tissue with Cd70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of mouse 1) spleen, 2) thymus, 3) lung, 4) RAW264.7, 5) Ana-1 and 6) SP2/0 cell lysate with Cd70 antibody. Predicted molecular weight ~21 kDa but may be observed at up to ~80 kDa due to glycosylation.
Western blot testing of 1) rat spleen, 2) rat thymus, 3) rat lung, 4) rat L6, 5) rat PC-12 and 6) mouse B16 cell lysate with Cd70 antibody. Predicted molecular weight ~21 kDa but may be observed at up to ~80 kDa due to glycosylation.
IHC staining of FFPE mouse spleen tissue with Cd70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse lymph node tissue with Cd70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cd70 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CD70 (Cluster of Differentiation 70) is a protein that in humans is encoded by CD70 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse Cd70 protein (amino acids C36-Q191) was used as the immunogen for the Cd70 antibody.
Limitation:
This Cd70 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O55237