TRPV1 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6968
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6968-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the TRPV1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human T-47D cells with TRPV1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human 293T, 2) HepG2, 3) rat liver and 4) rat PC-12 cell lysate with TRPV1 antibody. Predicted molecular weight ~95 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with TRPV1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRPV1 antibody.
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Immunofluorescent staining of FFPE human T-47D cells with TRPV1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) HepG2, 3) rat liver and 4) rat PC-12 cell lysate with TRPV1 antibody. Predicted molecular weight ~95 kDa.
Flow cytometry testing of human U-87 MG cells with TRPV1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRPV1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TRPV1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The transient receptor potential cation channel, subfamily V, member 1(TRPV1), also known as the capsaicin receptor is a protein which in humans is encoded by the TRPV1 gene. TRPV1(also called Vanilloid receptor type 1) is a ligand-gated nonselective cation channel that is considered to be an important integrator of various pain stimuli such as endogenous lipids, capsaicin, heat, and low pH. In addition to expression in primary afferents, TRPV1 is also expressed in the CNS. Cui M et al.(2006) demonstrate that TRPV1 receptors in the CNS plays an important role in pain mediated by central sensitization. And the significant CNS penetration is necessary for a TRPV1 antagonist to produce broad-spectrum analgesia. And TRPV1 also participates in normal bladder function and is essential for normal mechanically evoked purinergic signaling by the urothelium.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human TRPV1 protein (amino acids E107-A800) was used as the immunogen for the TRPV1 antibody.
Limitation:
This TRPV1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q8NER1