DAB2 Antibody / Disabled homolog 2 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6971
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6971-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DAB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human HeLa cells with DAB2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human melanoma tissue with DAB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human Caco-2, 4) monkey COS-7, 5) rat kidney, 6) rat NRK, 7) mouse kidney ad 8) mouse NIH 3T3 cell lysate with DAB2 antibody. Predicted molecular weight ~82 kDa but isoforms may be observed at approx. 69-96 kDa.
4 / 4
Flow cytometry testing of human HepG2 cells with DAB2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DAB2 antibody.
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Immunofluorescent staining of FFPE human HeLa cells with DAB2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human melanoma tissue with DAB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human Caco-2, 4) monkey COS-7, 5) rat kidney, 6) rat NRK, 7) mouse kidney ad 8) mouse NIH 3T3 cell lysate with DAB2 antibody. Predicted molecular weight ~82 kDa but isoforms may be observed at approx. 69-96 kDa.
Flow cytometry testing of human HepG2 cells with DAB2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DAB2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DAB2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Disabled homolog 2 is a protein that in humans is encoded by the DAB2 gene. This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H89-A770) was used as the immunogen for the DAB2 antibody.
Limitation:
This DAB2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P98082