E2F3 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6977
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6977-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the E2F transcription factor 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human liver cancer tissue with E2F transcription factor 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) human 293T, 2) human SH-SY5Y, 3) rat lung, 4) rat brain, 5) rat spleen and 6) mouse brain lysate with E2F transcription factor 3 antibody. Predicted molecular weight: ~49 kDa but can also be observed from 56~66 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with E2F transcription factor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= E2F transcription factor 3 antibody.
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IHC staining of FFPE human liver cancer tissue with E2F transcription factor 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293T, 2) human SH-SY5Y, 3) rat lung, 4) rat brain, 5) rat spleen and 6) mouse brain lysate with E2F transcription factor 3 antibody. Predicted molecular weight: ~49 kDa but can also be observed from 56~66 kDa.
Flow cytometry testing of human HL60 cells with E2F transcription factor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= E2F transcription factor 3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the E2F transcription factor 3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A91-S465) was used as the immunogen for the E2F transcription factor 3 antibody.
Limitation:
This E2F transcription factor 3 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O00716