EGR1 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6978
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6978-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Early growth response protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human K562 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
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Flow cytometry testing of mouse RAW264.7 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
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Flow cytometry testing of rat RH35 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
4 / 4
Western blot testing of 1) human K562, 2) human MCF-7, 3) human U-937, 4) human Jurkat, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse RAW264.7 cell lysate with Early growth response protein 1 antibody. Predicted molecular weight: 57 kDa (monomer), ~110 kDa (cytoplasmic dimer), ~75 kDa (nuclear dimer).
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Flow cytometry testing of human K562 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
Flow cytometry testing of mouse RAW264.7 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
Flow cytometry testing of rat RH35 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
Western blot testing of 1) human K562, 2) human MCF-7, 3) human U-937, 4) human Jurkat, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse RAW264.7 cell lysate with Early growth response protein 1 antibody. Predicted molecular weight: 57 kDa (monomer), ~110 kDa (cytoplasmic dimer), ~75 kDa (nuclear dimer).

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Early growth response protein 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Early growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a cancer suppressor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A2-M38) was used as the immunogen for the Early growth response protein 1 antibody.
Limitation:
This Early growth response protein 1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P18146