EIF3E Antibody [Clone 10F5H6] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6979
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6979-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 10F5H6
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the eIF-3 p48 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-2 OS cells with eIF-3 p48 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= eIF-3 p48 antibody.~
2 / 2
Western blot testing of 1) human Raji, 2) human K562, 3) rat thymu
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Flow cytometry testing of human U-2 OS cells with eIF-3 p48 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= eIF-3 p48 antibody.~
Western blot testing of 1) human Raji, 2) human K562, 3) rat thymu

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the eIF-3 p48 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A160-Q241) was used as the immunogen for the eIF-3 p48 antibody.
Limitation:
This eIF-3 p48 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P60228