EphA3 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6985
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6985-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Eph receptor A3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human PC-3 cells with Eph receptor A3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human placental tissue with Eph receptor A3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) MOLT4 and 2) placental tissue lysate with Eph receptor A3 antibody. Predicted molecular weight: 110/61 kDa (isoforms 1/2).
4 / 4
Flow cytometry testing of human PC-3 cells with Eph receptor A3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph receptor A3 antibody.
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Immunofluorescent staining of FFPE human PC-3 cells with Eph receptor A3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with Eph receptor A3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) MOLT4 and 2) placental tissue lysate with Eph receptor A3 antibody. Predicted molecular weight: 110/61 kDa (isoforms 1/2).
Flow cytometry testing of human PC-3 cells with Eph receptor A3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph receptor A3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Eph receptor A3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ephrin Receptor EphA3, is also known as human embryo kinase(HEK) or Eph-like tyrosine kinase1(ETK1). Kinases that phosphorylate proteins on tyrosine residues(protein tyrosine kinases; PTKs), such as EPHA3, form a structurally related group of molecules that exhibit functional diversity. Genetic alterations that lead to the inappropriate activation or expression of PTKs may be oncogenic. Many growth factor receptors are PTKs, e.g., the receptors for epidermal growth factor(EGFR), platelet-derived growth factor(PDGFR1;PDGFR2), colony-stimulating factor-1(CSF1R), and stem cell growth factor. The EPHA3 gene is mapped to 3p11.2. Sequence comparison with other PTKs showed a high degree of homology with members of the EPH and ELK(EPHB1) families of receptor tyrosine kinases. Within axial nerves, establishment of discrete afferent and efferent pathways depends on coordinate signaling between coextending sensory and motor projections.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D59-A514) was used as the immunogen for the Eph receptor A3 antibody.
Limitation:
This Eph receptor A3 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P29320