FEN1 Antibody / Flap endonuclease 1 [Clone 7D11D7] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6988
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6988-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 7D11D7
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FEN1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human ovarian cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human liver cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human colorectal adenocarcinoma tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human endometrial cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human MCF7 cells with FEN1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 9
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human MOLT4, 4) mouse thymus and 5) mouse RAW264.7 cell lysate with FEN-1 antibody. Expected molecular weight ~45 kDa.
9 / 9
Flow cytometry testing of human A431 cells with FEN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FEN1 antibody.
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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial cancer tissue with FEN1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with FEN1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human MOLT4, 4) mouse thymus and 5) mouse RAW264.7 cell lysate with FEN-1 antibody. Expected molecular weight ~45 kDa.
Flow cytometry testing of human A431 cells with FEN1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FEN1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the FEN1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human FEN1 protein (amino acids Q4-E300) was used as the immunogen for the FEN1 antibody.
Limitation:
This FEN1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P39748