GPBAR1 Antibody / TGR5 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6993
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6993-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the TGR5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human ThP-1 cells with TGR5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TGR5 antibody.~
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Western blot testing of 1) rat PC-12 and 2) mouse RAW264.7 cell lysate with T
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Flow cytometry testing of human ThP-1 cells with TGR5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TGR5 antibody.~
Western blot testing of 1) rat PC-12 and 2) mouse RAW264.7 cell lysate with T

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TGR5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The G protein-coupled bile acid receptor 1 (GPBAR1) also known G-protein coupled receptor 19 (GPCR19), membrane-type receptor for bile acids (M-BAR) and TGR5 as is a protein that in humans is encoded by the GPBAR1 gene. This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N76-S310) was used as the immunogen for the TGR5 antibody.
Limitation:
This TGR5 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q8TDU6