HGD Antibody / Homogentisate 1,2-dioxygenase [Clone 2F11E1] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6996
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6996-100ug100 ug£535.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2F11E1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Homogentisate 1,2-dioxygenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human liver cancer tissue with Homogentisate 1,2-dioxygenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human HCCP cell lysate with Homogentisate 1,2-dioxygenase antibody. Predicted molecular weight ~50 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with Homogentisate 1,2-dioxygenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Homogentisate 1,2-dioxygenase antibody.
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IHC staining of FFPE human liver cancer tissue with Homogentisate 1,2-dioxygenase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human HCCP cell lysate with Homogentisate 1,2-dioxygenase antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human Caco-2 cells with Homogentisate 1,2-dioxygenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Homogentisate 1,2-dioxygenase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Homogentisate 1,2-dioxygenase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The HGD gene encodes homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D374-N445) was used as the immunogen for the Homogentisate 1,2-dioxygenase antibody.
Limitation:
This Homogentisate 1,2-dioxygenase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q93099