PRMT3 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ6998
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6998-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PRMT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human Caco-2 cells with PRMT3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) MCF-7, 2) HepG2, 3) HeLa, 4) 293T, 5) K562, 6) Daudi, 7) HL60 and 8) MOLT4 cell lysate with PRMT3 antibody. Predicted molecular weight: 55-60 kDa.
3 / 3
Flow cytometry testing of human K562 cells with PRMT3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRMT3 antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with PRMT3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) MCF-7, 2) HepG2, 3) HeLa, 4) 293T, 5) K562, 6) Daudi, 7) HL60 and 8) MOLT4 cell lysate with PRMT3 antibody. Predicted molecular weight: 55-60 kDa.
Flow cytometry testing of human K562 cells with PRMT3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRMT3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PRMT3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein arginine N-methyltransferase 3 is an enzyme that in humans is encoded by the PRMT3 gene. This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R12-S514) was used as the immunogen for the PRMT3 antibody.
Limitation:
This PRMT3 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O60678