ICAM1 Antibody / CD54 [Clone 6F2C3] (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ7002
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7002-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 6F2C3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Intercellular adhesion molecule 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human rectal moderately differentiated adenocarcinoma tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human renal carcinoma tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human spleen tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) Raji, 2) HeLa, 3) K562 and 4) HepG2 cell lysate with Intercellular adhesion molecule 1 antibody. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
6 / 6
Flow cytometry testing of human Caco-2 cells with Intercellular adhesion molecule 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Intercellular adhesion molecule 1 antibody.

IHC staining of FFPE human rectal moderately differentiated adenocarcinoma tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal carcinoma tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with Intercellular adhesion molecule 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Raji, 2) HeLa, 3) K562 and 4) HepG2 cell lysate with Intercellular adhesion molecule 1 antibody. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
Flow cytometry testing of human Caco-2 cells with Intercellular adhesion molecule 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Intercellular adhesion molecule 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Intercellular adhesion molecule 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CD54, also known as ICAM-1 and Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q28-R268) was used as the immunogen for the Intercellular adhesion molecule 1 antibody.
Limitation:
This Intercellular adhesion molecule 1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P05362