RAB5 Antibody / RAB5A/B/C (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7003
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7003-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB5A/B/C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with RAB5A/B/C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC staining of FFPE human lymphoma tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human tonsil tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human lung cancer tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human adenocarcinoma of the right colon tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE human renal cell carcinoma tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
Western blot testing of 1) human U-87 MG, 2) human MCF7, 3) human U-251, 4) human HL60, 5) rat kidney, 6) rat testis and 7) mouse kidney tissue lysate with RAB5A/B/C antibody. Predicted molecular weight: 23-25 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with RAB5A/B/C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lymphoma tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adenocarcinoma of the right colon tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human MCF7, 3) human U-251, 4) human HL60, 5) rat kidney, 6) rat testis and 7) mouse kidney tissue lysate with RAB5A/B/C antibody. Predicted molecular weight: 23-25 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RAB5A/B/C antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human RAB5A/B/C protein (amino acids M1-N215) was used as the immunogen for the RAB5A/B/C antibody.
Limitation:
This RAB5A/B/C antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P20339, P61020, P51148