c-Jun Antibody / JUN (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7005
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7005-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the c-Jun antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human A431 cells with c-Jun antibody (green) and Beta Tubulin antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human renal pelvis squamous metaplasia tissue with c-Jun antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human renal cell carcinoma tissue with c-Jun antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) HeLa, 2) K562, 3) Jurkat and 4) U-87 MG cell lysate with c-Jun antibody. Predicted molecular weight ~36 kDa.
5 / 5
Flow cytometry testing of human U-2 OS cells with c-Jun antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= c-Jun antibody.
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Immunofluorescent staining of FFPE human A431 cells with c-Jun antibody (green) and Beta Tubulin antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human renal pelvis squamous metaplasia tissue with c-Jun antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma tissue with c-Jun antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) K562, 3) Jurkat and 4) U-87 MG cell lysate with c-Jun antibody. Predicted molecular weight ~36 kDa.
Flow cytometry testing of human U-2 OS cells with c-Jun antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= c-Jun antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the c-Jun antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
c-Jun is a protein that in humans is encoded by the JUN gene. This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K35-F331) was used as the immunogen for the c-Jun antibody.
Limitation:
This c-Jun antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P05412