MSH2 Antibody [Clone 6B4F7] (Antigen affinity purified)

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ7009
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7009-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 6B4F7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MSH2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human serous adenocarcinoma of the ovary tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human liver cancer tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Immunofluorescent staining of FFPE human Caco-2 cells with MSH2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 5
Western blot testing of human 1) HeLa, 2) MCF7, 3) A459 and 4) COLO-320 cell lysate with MSH2 antibody. Expected molecular weight ~105 kDa.
❮❯

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human serous adenocarcinoma of the ovary tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with MSH2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with MSH2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) MCF7, 3) A459 and 4) COLO-320 cell lysate with MSH2 antibody. Expected molecular weight ~105 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the MSH2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
DNA mismatch repair protein Msh2, also known as MutS protein homolog 2 or MSH2, is a protein that in humans is encoded by the MSH2 gene, which is located on chromosome 2. MSH2 is a tumor suppressor gene and more specifically a caretaker gene that codes for a DNA mismatch repair (MMR) protein, MSH2 which forms a heterodimer with MSH6 to make the human MutS-alpha mismatch repair complex. It also dimerizes with MSH3 to form the MutS-beta DNA repair complex. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. It has been found that MSH2 may also be a coactivator of ESR1-dependent gene expression.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human MSH2 protein (amino acids Q337-N583) was used as the immunogen for the MSH2 antibody.
Limitation:
This MSH2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P43246