MTCO2 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7011
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7011-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the COX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat liver, 2) rat brain, 3) rat small intestine, 4) rat RH35, 5) mouse liver, 6) mouse brain, 7) mouse small intestine and 8) mouse Neuro-2a cell lysate with COX2 antibody. Predicted molecular weight ~25 kDa.
2 / 7
Western blot testing of human 1) K562, 2) MCF7, 3) HeLa, 4) Jurkat, 5) A431 and 6) HaCaT cell lysate with COX2 antibody. Predicted molecular weight ~25 kDa.
3 / 7
IHC staining of FFPE human colorectal cancer tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human glioma tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE mouse brain tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE rat brain tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
Flow cytometry testing of human U-87 MG cells with COX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COX2 antibody.
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Western blot testing of 1) rat liver, 2) rat brain, 3) rat small intestine, 4) rat RH35, 5) mouse liver, 6) mouse brain, 7) mouse small intestine and 8) mouse Neuro-2a cell lysate with COX2 antibody. Predicted molecular weight ~25 kDa.
Western blot testing of human 1) K562, 2) MCF7, 3) HeLa, 4) Jurkat, 5) A431 and 6) HaCaT cell lysate with COX2 antibody. Predicted molecular weight ~25 kDa.
IHC staining of FFPE human colorectal cancer tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioma tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with COX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human U-87 MG cells with COX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COX2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the COX2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (called COX2, COII and MT-CO2) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-S205) was used as the immunogen for the COX2 antibody.
Limitation:
This COX2 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
P00403