Neuropilin 1 Antibody / NRP1 [Clone 4G3F7] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7016
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7016-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 4G3F7
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Neuropilin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A549 cells with Neuropilin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human PC-3, 2) human U-87 MG, 3) rat brain and 4) rat C6 cell lysate with Neuropilin 1 antibody. Expected molecular weight: 102-130 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with Neuropilin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neuropilin 1 antibody.
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Immunofluorescent staining of FFPE human A549 cells with Neuropilin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human PC-3, 2) human U-87 MG, 3) rat brain and 4) rat C6 cell lysate with Neuropilin 1 antibody. Expected molecular weight: 102-130 kDa.
Flow cytometry testing of human U-87 MG cells with Neuropilin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neuropilin 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Neuropilin 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K504-T827) was used as the immunogen for the Neuropilin 1 antibody.
Limitation:
This Neuropilin 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O14786