Pleckstrin Antibody / PLEK (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7023
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7023-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PLEK antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human tonsil tissue with PLEK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) HEL and 2) U-937 cell lysate with PLEK antibody. Predicted molecular weight: ~40 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with PLEK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLEK antibody.
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IHC staining of FFPE human tonsil tissue with PLEK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEL and 2) U-937 cell lysate with PLEK antibody. Predicted molecular weight: ~40 kDa.
Flow cytometry testing of human HL60 cells with PLEK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLEK antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PLEK antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables phosphatidylinositol-3,4-bisphosphate binding activity; protein homodimerization activity; and protein kinase C binding activity. Involved in several processes, including G protein-coupled receptor signaling pathway; actin cytoskeleton organization; and positive regulation of supramolecular fiber organization. Located in cytoplasm and ruffle membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M47-K308) was used as the immunogen for the PLEK antibody.
Limitation:
This PLEK antibody is available for research use only.
Localization:
Cytoplasmic, extracellular
Purity:
Antigen affinity purified
Uniprot #:
P08567