PRKRA Antibody / PACT (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7025
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7025-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PACT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE mouse brain tissue with PACT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat brain tissue with PACT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HeLa, 2) human K562, 3) human HepG2, 4) rat testis, 5) rat PC-12 and 6) mouse testis tissue with PACT antibody. Predicted molecular weight: 32-34 kDa.
4 / 4
Flow cytometry testing of human K562 cells with PACT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PACT antibody.
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IHC staining of FFPE mouse brain tissue with PACT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with PACT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human K562, 3) human HepG2, 4) rat testis, 5) rat PC-12 and 6) mouse testis tissue with PACT antibody. Predicted molecular weight: 32-34 kDa.
Flow cytometry testing of human K562 cells with PACT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PACT antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PACT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein kinase, interferon-inducible double stranded RNA dependent activator, also known as interferon-inducible double stranded RNA-dependent protein kinase activator A or Protein ACTivator of the interferon-induced protein kinase (PACT) is a protein that in humans is encoded by the PRKRA gene. This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A154-K313) was used as the immunogen for the PACT antibody.
Limitation:
This PACT antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O75569