p23 Antibody / PTGES3 [Clone 9D3D1] (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7027
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7027-100ug100 ug£535.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 9D3D1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Prostaglandin E synthase 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human T-47D cells with Prostaglandin E synthase 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human COLO-320, 4) human K562, 5) rat liver, 6) rat RH35 and 7) mouse HEPA1-6 cell lysate with Prostaglandin E synthase 3 antibody. Predicted molecular weight ~23 kDa.
3 / 3
Flow cytometry testing of human PC-3 cells with Prostaglandin E synthase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prostaglandin E synthase 3 antibody.
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Immunofluorescent staining of FFPE human T-47D cells with Prostaglandin E synthase 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human COLO-320, 4) human K562, 5) rat liver, 6) rat RH35 and 7) mouse HEPA1-6 cell lysate with Prostaglandin E synthase 3 antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human PC-3 cells with Prostaglandin E synthase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prostaglandin E synthase 3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Prostaglandin E synthase 3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Prostaglandin E synthase 3 (cytosolic) is an enzyme that in humans is encoded by the PTGES3 gene. It is mapped to 12q13.3; 12. This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-K79) was used as the immunogen for the Prostaglandin E synthase 3 antibody.
Limitation:
This Prostaglandin E synthase 3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q15185