Poliovirus Receptor Antibody / PVR [Clone 9B9F1] (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ7029
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7029-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 9B9F1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Poliovirus Receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE human liver cancer tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 7
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human endometrial cancer tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human colorectal adenocarcinoma tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human ovarian tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE mouse liver tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
Western blot testing of human 1) A431, 2) A549, 3) HT1080 and 4) Daudi cell lysate with Poliovirus Receptor antibody. Predicted molecular weight ~45 kDa, but may be observed at higher molecular weights due to glycosylation.

IHC staining of FFPE human liver cancer tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial cancer tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver tissue with Poliovirus Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A431, 2) A549, 3) HT1080 and 4) Daudi cell lysate with Poliovirus Receptor antibody. Predicted molecular weight ~45 kDa, but may be observed at higher molecular weights due to glycosylation.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
Optimal dilution of the Poliovirus Receptor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D28-E331) was used as the immunogen for the Poliovirus Receptor antibody.
Limitation:
This Poliovirus Receptor antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P15151