RANGAP1 Antibody (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7033
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7033-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Ran GTPase-activating protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) rat brain, 2) rat stomach, 3) rat small intestine, 4) rat pancreas, 5) mouse brain, 6) mouse stomach, 7) mouse small intestine and 8) mouse pancreas tissue lysate with Ran GTPase-activating protein 1 antibody. Predicted molecular weight 64-70 kDa.
2 / 5
Western blot testing of human 1) SH-SY5Y, 2) 293T, 3) HeLa, 4) A431, 5) HaCaT, 6) MCF7, 7) HL60 and 8) U-251 cell lysate with Ran GTPase-activating protein 1 antibody. Predicted molecular weight 64-70 kDa.
3 / 5
IHC staining of FFPE human colorectal carcinoma tissue with Ran GTPase-activating protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Immunofluorescent staining of FFPE human A431 cells with Ran GTPase-activating protein 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 5
Flow cytometry testing of human U-87 MG cells with Ran GTPase-activating protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ran GTPase-activating protein 1 antibody.
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Western blot testing of 1) rat brain, 2) rat stomach, 3) rat small intestine, 4) rat pancreas, 5) mouse brain, 6) mouse stomach, 7) mouse small intestine and 8) mouse pancreas tissue lysate with Ran GTPase-activating protein 1 antibody. Predicted molecular weight 64-70 kDa.
Western blot testing of human 1) SH-SY5Y, 2) 293T, 3) HeLa, 4) A431, 5) HaCaT, 6) MCF7, 7) HL60 and 8) U-251 cell lysate with Ran GTPase-activating protein 1 antibody. Predicted molecular weight 64-70 kDa.
IHC staining of FFPE human colorectal carcinoma tissue with Ran GTPase-activating protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with Ran GTPase-activating protein 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-87 MG cells with Ran GTPase-activating protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ran GTPase-activating protein 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ran GTPase-activating protein 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ran GTPase-activating protein 1 is an enzyme that in humans is encoded by the RANGAP1 gene. This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A165-V587) was used as the immunogen for the Ran GTPase-activating protein 1 antibody.
Limitation:
This Ran GTPase-activating protein 1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P46060