CENPI Antibody / Centromere protein I (Antigen affinity purified)

NSJ Bioreagents
Product Code: NSJ-RQ7043
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7043-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the CENPI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U937 cells with CENPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CENPI antibody.~
2 / 2
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) hu

Flow cytometry testing of human U937 cells with CENPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CENPI antibody.~
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) hu

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CENPI antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Centromere protein I is a protein that in humans is encoded by the CENPI gene. This gene encodes a centromere protein that is a component of the CENPA-NAC (nucleosome-associated) complex. This complex is critical for accurate chromosome alignment and segregation and it ensures proper mitotic progression. This protein regulates the recruitment of kinetochore-associated proteins that are required to generate the spindle checkpoint signal. The product of this gene is involved in the response of gonadal tissues to follicle-stimulating hormone. Mutations in this gene may be involved in human X-linked disorders of gonadal development and gametogenesis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 13.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human CENPI protein (amino acids K120-C604) was used as the immunogen for the CENPI antibody.
Limitation:
This CENPI antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q92674