TP53BP1 Antibody / TP53-binding protein 1 (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7044
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7044-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the p53 binding protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human bladder epithelial carcinoma tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human metaplasia of squamous cells of the renal pelvis tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human ovarian cancer tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human moderately differentiated rectal adenocarcinoma tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human placental tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human placental tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Immunofluorescent staining of FFPE human HeLa cells with p53 binding protein 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 11
Western blot testing of human U-2 OS cells with p53 binding protein 1 antibody. Predicted molecular weight: ~214 kDa but may be observed at up to ~450 kDa.
11 / 11
Flow cytometry testing of human HeLa cells with p53 binding protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p53 binding protein 1 antibody.
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IHC staining of FFPE human breast cancer tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder epithelial carcinoma tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human metaplasia of squamous cells of the renal pelvis tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human moderately differentiated rectal adenocarcinoma tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with p53 binding protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with p53 binding protein 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human U-2 OS cells with p53 binding protein 1 antibody. Predicted molecular weight: ~214 kDa but may be observed at up to ~450 kDa.
Flow cytometry testing of human HeLa cells with p53 binding protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p53 binding protein 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the p53 binding protein 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
TP53BP1 (Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H1239-H1972) was used as the immunogen for the p53 binding protein 1 antibody.
Limitation:
This p53 binding protein 1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q12888