ACAT2 Antibody / Acetyl-CoA acetyltransferase, cytosolic (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7052
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7052-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ACAT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HepG2 cells with ACAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACAT2 antibody.~
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Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Jurkat, 4)
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Flow cytometry testing of human HepG2 cells with ACAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACAT2 antibody.~
Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Jurkat, 4)

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ACAT2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT2 (acetyl-Coenzyme A acetyltransferase 2) gene. The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q31-E397) was used as the immunogen for the ACAT2 antibody.
Limitation:
This ACAT2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9BWD1